Electrophilic aromatic substituted luciferins as bioluminescent probes for glutathione S-transferase assays.

نویسندگان

  • Wenhui Zhou
  • John W Shultz
  • Nancy Murphy
  • Erika M Hawkins
  • Laurent Bernad
  • Troy Good
  • Leonard Moothart
  • Susan Frackman
  • Dieter H Klaubert
  • Robert F Bulleit
  • Keith V Wood
چکیده

New highly sensitive latent bioluminescent luciferin substrates were designed and synthesized for monitoring mammalian glutathione S-transferase (GST) and Schistosoma japonicum enzyme activities.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Glutathione S-transferase A. A novel kinetic mechanism in which the major reaction pathway depends on substrate concentration.

Glutathione transferase A has been purified from rat liver. The enzyme catalyzes the conjugation of glutathione with compounds bearing an electrophilic site, especially those in which the electrophilic site is on, or cr to, an aromatic ring. The enzyme has a molecular weight of 45,000 and is composed of two similar subunits. Initial velocity, product inhibition, and binding studies indicate a b...

متن کامل

Electrophilic probes for deciphering substrate recognition by O-GlcNAc transferase.

O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) is an essential human glycosyltransferase that adds O-GlcNAc modifications to numerous proteins. However, little is known about the mechanism with which OGT recognizes various protein substrates. Here we report on GlcNAc electrophilic probes (GEPs) to expedite the characterization of OGT-substrate recognition. Data from mass spectromet...

متن کامل

Xenobiotic-inducible expression of murine glutathione S-transferase Ya subunit gene is controlled by an electrophile-responsive element.

Glutathione S-transferase (GST) Ya subunit gene expression is induced in mammalian tissues by two types of chemical agents: (i) planar aromatic compounds (e.g., 3-methylcholanthrene, beta-naphthoflavone, and 2,3,7,8-tetrachlorodibenzo-p- dioxin) and (ii) electrophiles (e.g., trans-4-phenyl-3-buten-2-one and dimethyl fumarate) or compounds easily oxidized to electrophiles (e.g., tert-butylhydroq...

متن کامل

Production and Evaluation of Polyclonal Rabbit Anti-Human p53 Antibody Using Bacterially Expressed Glutathione S-transferase-p53 fusion protein

p53 is a key tumor suppressor gene that is targeted for inactivation during human tumorigenesis. In this study, we produced and characterized polyclonal antihuman p53 antibody. The cDNA encoding the completehuman p53 protein was cloned into pGEX-4T-1 and expressed in Escherichia coli as a fusion protein with Schistosoma japonicum glutathione S-transferase (GST). The rabbits were immunized...

متن کامل

random-order sequential mechanism

Under standard assay conditions, with 1-chloro-2,4-dinitrobenzene (CDNB) as electrophilic substrate, rat glutathione transferase 4-4 is strongly inhibited (I50= 1 PM) by indomethacin. No other glutathione transferase investigated is significantly inhibited by micromolar concentrations of indomethacin. Paradoxically, the strong inhibition of glutathione transferase 44 was dependent on high (mill...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Chemical communications

دوره 44  شماره 

صفحات  -

تاریخ انتشار 2006